Journal: Cell metabolism

Article Title: SIRT4 is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion

doi: 10.1016/j.cmet.2017.03.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The membranes were incubated with streptavidin-AlexaFluor 680 (Thermo Fisher Scientific, # {"type":"entrez-protein","attrs":{"text":"S32358","term_id":"422838","term_text":"pir||S32358"}} S32358 ) at a dilution of 1/2000 in LI-COR buffer/Tween for 1 h, washed, and the signal imaged and quantified using the LI-COR Odyssey Infrared Imaging System.

Techniques: Recombinant, Protein Extraction, Lysis, Produced, Mutagenesis, Staining, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Protease Inhibitor, Plasmid Preparation, Software, Sample Prep, Imaging, Thin Layer Chromatography

Journal: bioRxiv

Article Title: A mRNA-LNP vaccine against Dengue Virus elicits robust, serotype-specific immunity

doi: 10.1101/2021.01.05.425517

Figure Lengend Snippet: A) Schematic of the DENV genome and engineered mRNA construct. An mRNA encoding for the prM and ENV viral proteins was engineered with N-terminal signal peptide sequence, 5’ and 3’ untranslated regions (UTR) flanking the coding sequence, a 3’ poly-A tail, and a 5’ cap-1 structure. In vitro synthesized mRNA is encapsulated in a lipid nanoparticle for use in in vitro and in vivo experiments. B) 293T cells were transfected with the in vitro transcribed mRNA encoding for the wild-type sequence (WT), or a mutant version with amino acid substitutions in the fusion-loop epitope (ΔFL). Lysate was analyzed by western blot with the domain III specific 1A1D-2 monoclonal antibody and the fusion-loop specific 4G2 monoclonal antibody. C) Supernatant from transfected cells was purified and concentrated through ultracentrifugation and analyzed for VLPs by western blots with the 1A1D-2 monoclonal antibody or anti-GAPDH. Unpurified cell lysate from WT mRNA transfected cells is included as a control. Shown are representative blots. D) Electron microscopy image of VLPs from purified supernatant of transfected 293T cells showing homogenous shape and size of approximately 30nm.

Article Snippet: Membranes were blotted with envelope domain III specific 1A1D-2 (1:600) monoclonal antibody (CDC Arbovirus Reference Collection) or envelope fusion-loop specific 4G2 (3.33 mg/ml) (BEI Cat# NR-50327, Novus Biologicals Cat# NBP2-52709FR).

Techniques: Construct, Sequencing, In Vitro, Synthesized, In Vivo, Transfection, Mutagenesis, Western Blot, Purification, Control, Electron Microscopy

Journal: Acta Physica Polonica A

Article Title: Mechanical Activation of Deposited Brown Coal Fly Ash in Stirred Media Mill

doi: 10.12693/aphyspola.126.988

Figure Lengend Snippet: Fig. 6. FT-IR spectra of ground y ash (vcir = 5 m/s).

Article Snippet: Investigation was carried out using a JASCO FT-IR 4200 type Fourier transform infrared spectrometer, in transmission mode, on KBr cemented pellets.

Techniques:

Journal: Acta Physica Polonica A

Article Title: Mechanical Activation of Deposited Brown Coal Fly Ash in Stirred Media Mill

doi: 10.12693/aphyspola.126.988

Figure Lengend Snippet: Fig. 7. FT-IR spectra of ground y ash (vcir = 7 m/s).

Article Snippet: Investigation was carried out using a JASCO FT-IR 4200 type Fourier transform infrared spectrometer, in transmission mode, on KBr cemented pellets.

Techniques:

Journal: Journal of AOAC International

Article Title: Validation of the PhageDx™ Listeria Assay for Detection of Listeria Spp. on Stainless Steel and Ceramic Environmental Surfaces AOAC Performance Tested Method SM 102005

doi: 10.1093/jaoacint/qsab036

Figure Lengend Snippet: PhageDx Listeria inclusivity list

Article Snippet: L. innocua (ATCC BAA-680) and L. welshimeri (ATCC 43551) were detected with 20 h enrichment, or within the recommended enrichment time frame for environmental samples (data not shown).

Techniques: Environmental Sampling

Journal: Journal of AOAC International

Article Title: Validation of the PhageDx™ Listeria Assay for Detection of Listeria Spp. on Stainless Steel and Ceramic Environmental Surfaces AOAC Performance Tested Method SM 102005

doi: 10.1093/jaoacint/qsab036

Figure Lengend Snippet: PhageDx Listeria results: presumptive vs confirmed using the reference method procedure

Article Snippet: L. innocua (ATCC BAA-680) and L. welshimeri (ATCC 43551) were detected with 20 h enrichment, or within the recommended enrichment time frame for environmental samples (data not shown).

Techniques:

Journal: Journal of AOAC International

Article Title: Validation of the PhageDx™ Listeria Assay for Detection of Listeria Spp. on Stainless Steel and Ceramic Environmental Surfaces AOAC Performance Tested Method SM 102005

doi: 10.1093/jaoacint/qsab036

Figure Lengend Snippet: Method comparison results: PhageDx Listeria vs BAM Ch. 10

Article Snippet: L. innocua (ATCC BAA-680) and L. welshimeri (ATCC 43551) were detected with 20 h enrichment, or within the recommended enrichment time frame for environmental samples (data not shown).

Techniques: Comparison

Journal: Journal of AOAC International

Article Title: Validation of the PhageDx™ Listeria Assay for Detection of Listeria Spp. on Stainless Steel and Ceramic Environmental Surfaces AOAC Performance Tested Method SM 102005

doi: 10.1093/jaoacint/qsab036

Figure Lengend Snippet: PhageDx Listeria results: presumptive vs confirmed using the PhageDx Listeria confirmation procedure

Article Snippet: L. innocua (ATCC BAA-680) and L. welshimeri (ATCC 43551) were detected with 20 h enrichment, or within the recommended enrichment time frame for environmental samples (data not shown).

Techniques:

Journal: Journal of AOAC International

Article Title: Validation of the PhageDx™ Listeria Assay for Detection of Listeria Spp. on Stainless Steel and Ceramic Environmental Surfaces AOAC Performance Tested Method SM 102005

doi: 10.1093/jaoacint/qsab036

Figure Lengend Snippet: Method comparison results: PhageDx Listeria vs BAM Ch. 10

Article Snippet: L. innocua (ATCC BAA-680) and L. welshimeri (ATCC 43551) were detected with 20 h enrichment, or within the recommended enrichment time frame for environmental samples (data not shown).

Techniques: Comparison